PO301 code for my E 350 Coupe Question?
Need 3 things for proper fire : fuel, air, compression. Assuming you have compression, leaves fuel and spark. Swap the coil pack (and boot) with one from another cylinder. If the misfire happens on the other cylinder then the coil pack and/or boot is the culprit.
If misfire remains in the same cylinder unplug the fuel injector at that cylinder. If no change in running then either harness/connector or injector itself has a problem. If engine runs worse that's good actually, it tells you that the fuel supply is probably ok. I use a homemade led light as a noid light to check if the harness is providing current pulses to the injector. A quick check also is to put a screwdriver or hose on the injector while running and listen if it's constantly clicking. But that's not telling you if the injector is clogged. Generally injectors last a long time but you never know.
Try the simple tests first.
Be sure all connectors and coil pack boots are secure and dielectric grease is used at plugs. Not that you will, but driving w flashing cel (misfire) will cause the ecu to move to a rich mixture. Not good for the catalytic converter or O2 sensors.


